PAUL G. YOUNG
cycle genetics and molecular biology
Paul Young's Homepage [homepage]
My interests revolve broadly around cell biology and
development in the fission yeast. Yeast offers the advantage of well-developed genetics
and ease of cloning of genes. Following the completion of a number of full genome
sequences including that of fission yeast, findings can often be rapidly translated to
mammalian or plant systems. The projects in the lab range widely from pure genetics to
molecular biology, biochemistry or digital imaging. The strength of a genetic system such
as yeast is the ability to move easily from one approach to another and to bring all to
bear on an interesting problem in biology.
My major area of interest is cell cycle control. Yeast systems have led in defining the basic paradigm of eukaryotic cell cycle control (Nobel prizes to P. Nurse, T. Hunt and L. Hartwell in 2001 for this work) and in continuing to find new elements in the puzzle. Many of the proteins discovered to play a role in yeast have their direct mammalian counterparts. Projects in the lab focus especially on the mitotic control and the way in which external information such as nutritional status bears on its regulation. In addition we are investigating checkpoint pathways which serve to arrest major events of the cell cycle when earlier events are incomplete or not executed properly.
A second area of interest is ion transport and pH regulation in cells. We have studied the sodium/proton antiporter from fission yeast and are currently involved in investigating related transporters and regulators. A recent interest is to examine pH regulation and its coupling (or lack of it) to the cell cycle and the way in which a cell establishes an internal pH setpoint.
Eid R., Arab, N. T., Boucher, E., Young, P. G., Zhou, D. R., Mandato, C. A., and Greenwood, M. (2017) Heterologous expression of anti-apoptotic human 14-3-3b/a enhances iron-mediated programmed cell death in yeast. Plos One 12:e084151 doi: 10.1371/journal.pone.08141.eCollection 2017
Young, P. G. 2017. Glimpses of a Cultured Scientist, in To the Beat of his Own Drum, Murdoch Mitchison, ed. S. Mitchison, pp 198-203.
Eid R., Boucher, E., Gharib, N., Arab, N. T.. Murray, A., Young, P. G., Mandato, C. A., and Greenwood, M. 2016. Identification of human ferritin, heavy polypeptide 1 (FTH1) and yeast RGI1 (YER067W) as pro-survival sequences that counteract the effects of Bax and copper in Saccharomyces cerevisiae. Exp. Cell Res., 342:52-61. doi: 10.1016/j.yexcr.2016.02.010.
Jones, N. K., Arab, N. T. T., Eid, R., Gharib, N., Sheibani, S., Vali, H., Khoury, C., Murray, A., Boucher, E., Mandato, C. A., Young, P. G., and Greenwood, M. T. 2015. Human Thyroid Cancer-1 (TC-1) is a vertebrate specific oncogenic protein that protects against copper and pro-apoptotic genes in yeast. Microb Cell. 6:247-255. doi: 10.15698/mic2015.07.213.
Shebani, S., Jones, N., Eid, R., Gharib, N., Titorenko, V., Vali, H., Young, P., and Greenwood, M. 2015. Inhibition of stress mediated cell death by human Lactate Dehydrogenase B (LDHB) in yeast. FEMS Yeast Res 15:fov132 doi: 10.193/femsyr/fov032.
Freitag, S.I., Wong, J. and Young, P. G. 2014. Genetic and physical interaction of Ssp1 CaMKK adn Rad24 14-3-3 during low pH and osmotic stress in fission yeast. OpenBiology 4, 130127. http://rsob.royalsocietypublishing.org/content/4/1/130127.
Frazer, C. and Young, P. G. 2012. Carboxyl-terminal phosphorylation sites in Cdc25 contribute to enforcement of the DNA damage and replication checkpoints in fission yeast. Curr Genet. 58, 217-34 (pubmed link)
Frazer, C. and Young, P.G. 2012. Phosphorylation mediated regulation of Cdc25 activity, localization and stability. Protein Phosphorylation in Human Health, Cai Huang (Ed.). ISBN: 978-953-51-0737-8, Intech. (link)
Park, J., Freitag, S., Young, P. G. and Hobman, T. C. 2012. The karyopherin Sal3 is required for nuclear import of the core RNA interference pathway protein Rdp1. Traffic, 13, 520-531. (pubmed link)
Frazer, C. and Young, P. G. 2011. Redundant mechanisms prevent mitotic entry following replication arrest in the absence of Cdc25 hyperphosphorylation. PLoSOne, 6:e21348 (pubmed link)
Salehi, M., Young, P. G. and Mousavi, P. 2008. Modeling of the transcriptional subnetwork in the yeast cell cycle pathway using dynamic bayesian networks and evolutionary search. Proceedings of the IEEE Conference on Computational Intelligence in Bioinformatics and Bioenginerring. Sunny Valley, ID, September 2008.
Benko Z, Liang D, Agbottah E, Hou J, Taricani L, Young PG, Bukrinsky
M, Zhao RY. 2007. Antagonistic interaction of HIV-1 Vpr with Hsf-mediated
cellular heat shock response and Hsp16 Activation in fission yeast (Schizosaccharomyces
pombe). Retrovirology 4,16 (pubmed
McQuire, T. and P. G. Young. 2006. Joint regulation of the nmt1 promoter and sporulation by Thi1 and Thi5 in Schizosaccharomyces pombe . Curr. Genet. 50, 269-279. (pubmed link)
Fliegel, L., C. Wiebe, G. Chua and P. G. Young. 2005. Functional expression and cellular localization of the Na+/H+ exchanger Sod2 of the fission yeast, Schizosaccharomyces pombe. Can. J. Physiol. Pharmacol. 83, 565-72. (pubmed link)
Benko, Z., D. Liang, E. Agbottah, J. Hou, K. Chiu, M. Yu, S. Innis, P. Reed, W. Kabat, R. T. Elder, P. Di Marzio, L. Taricani, L. Ratner, P. G. Young, M. Bukrinsky and Y. Zhao. 2004. Anti-Vpr activity of a yeast chaperone protein. J. Virology 78, 11016-11029.(pubmed link)
Fixen, W. D., Johnson, L., Merriam, J and Young, P. G. Solutions Megamanual for Introduction to Genetic Analysis, Eighth Edition, 2004. W. H. Freeman Inc.
Wiebe, C., C. Rieder, P.G. Young, P. Dibrov and L. Fliegel. 2003. Functional analysis of amino acids of the Na+/H+ exchanger that are important for proton translocation. Mol. Cell. Biochem. 254, 117-124. (pubmed link)
Young, P. G. 2003. Exploring Genomes. W. H. Freeman, N.Y.
Chua, G., C. Lingner, C. Frazer and P. G. Young. 2002. The sal3 gene encodes an importin-b implicated in the nuclear import of Cdc25 in Schizosaccharomyces pombe. Genetics 162, 689-703. (pubmed link)
Karagiannis, J., R. Oulton and P. G. Young. 2002. The Scw1 RNA binding domain protein is involved in the regulation of septation and cell wall structure in fission yeast. Genetics 162, 45-58. (pubmed link)
Rupes, I. and P. G. Young. 2002. Morphogenetic checkpoint in fission yeast? No! Microbiology 148, 2271-2272. (pubmed link)
Taricani, L., M. Tejada and P. G. Young. 2002. The fission yeast ES2 homologue, Bis1, interacts with the Ish1 stress-responsive nuclear envelope protein. J. Biol. Chem. 277, 10562-72. (pubmed link)
Rupes, I., B. A. Webb, A. Mak and P. G. Young. 2001. G2/M arrest caused by actin disruption is a
manifestation of the cell size checkpoint in fission yeast . Mol. Biol. Cell 12, 3892-3903.
Karagiannis, J. and P. G. Young. 2001. Intracellular pH homeostasis during cell cycle progression and growth state transition in Schizosaccharomyces pombe. J. Cell Sci. 114, 2929-2941. (pubmed link)
. Mol. Biol. Cell 12, 3892-3903. (pubmed link)
Taricani, L., H. Feilotter, C. Weaver and P.G. Young. 2001. Expression of hsp16 in Schizosaccharomyces pombe in response to nucleotide depletion is regulated via the spc1 MAP kinase pathway. Nucleic Acids Research 29, 3030-40. (pubmed link)
Chua, G., L. Taricani, W. Stangle and P. G. Young. 2000. Insertional mutagenesis based on illegitimate recombination in Schizosaccharomyces pombe. Nucleic Acids Research, 28, e53 (6 pages). (pubmed link)
Rupes, I., Z. Jia and P. G. Young. 1999. Ssp1 promotes actin depolymerization and is involved in stress response and NETO control in fission yeast. Mol. Biol. Cell 10, 1495-1510. (pubmed link)
Karagiannis, J., R. Saleki and P. G. Young. 1999. The pub1 E3 ubiquitin ligase negatively regulates leucine uptake in response to NH4+ in fission yeast. Curr. Genetics 35, 593-601. (pubmed link)
Dibrov, P., P. G. Young and L. Fliegel. 1998. Physiological consequences of expression of sod2 in Escherichia coli . Mol. Cell Biochem. 183, 125-132. (pubmed link)
Dibrov, P., P. G. Young and L. Fliegel. 1998. Functional analysis of amino acid residues essential for activity in the Na+/H+ exchanger of fission yeast. Biochemistry 37, 8282-8288. (pubmed link)
Breeding, C. S., J. Hudson, M. K. Balasubramanian, S. Hemmingsen, P. G. Young and K. L. Gould. 1998. The cdr2+ gene encodes a regulator of G2/M progression and cytokinesis in Schizosaccharomyces pombe. Mol. Biol. Cell 9, 3399-3415. (pubmed link)
Saleki, R., Z. Jia, J. Karagiannis and P. G. Young. 1997. Low pH tolerance in Schizosaccharomyces pombe requires a functioning pub1 ubiquitin ligase. Mol. Gen. Genetics 254, 520-528. (pubmed link)
Dibrov, P., J. J.. Smith, P. G. Young and L. Fliegel. 1997. Identification and localization of the sod2 gene product in Schizosaccharomyces pombe. FEBS Letts 405, 119-124. (pubmed link)
Rupes, I., J. Jochova and P. G. Young. 1997. Markers of cell polarity during and after nitrogen starvation in Schizosaccharomyces pombe. Biochem. Cell Biol. 75, 697-708. (pubmed link)
Hahnenberger, K.M., Z. Jia, L. Fliegel, S. Hemmingsen and P. G. Young. 1996. Sodium tolerance and export from yeast cells. In The Na+/H+ Exchanger. L. Fliegel, ed. Landes Co., U.S.A. (pubmed link)
Hahnenberger, K., Z. Jia and P. G. Young. 1996. Functional expression of the Schizosaccharomyces pombe Na+/H+ antiporter gene, sod2, in Saccharomyces cerevisiae. Proc. Nat. Acad. Sci., U.S.A. 93, 5031-5036. (pubmed link)
Saleki, R., Young, P. G. and Lefebvre, D. 1993. Mutants affected in sodium
tolerance for germination in Arabidopsis thaliana. Plant Physiology 101, 839-845.
Hudson, J.D. and P. G. Young. 1993. Sequence of the Schizosaccharomyces pombe gtp1 gene and the identification of a novel family of putative GTP binding proteins. Gene 25, 191-193. (pubmed link)
Jia, Z., N. McCullough, L. Wong and P. G. Young. 1993. The amiloride resistance gene car1 of Schizosaccharomyces pombe. Mol. Gen. Genet. 241, 298-304. (pubmed link)
Parker, L.L., S.A.Walker, P. G. Young and H. Piwnica-Worms. 1993. Phosphorylation and inactivation of the mitotic inhibitor wee1 by the nim1/cdr1 kinase. Nature 363, 736-738. (pubmed link)
Rowley, R., S. Subramani and P. G. Young. 1992. Checkpoint controls in Schizosaccharomyces pombe. EMBO J. 11, 1335-1342. (pubmed link)
Jia, Z., N. McCullough, R. Martel, S. Hemmingsen and P. G. Young. 1992. Gene amplification at a locus encoding a putative Na+/H+ antiport confers sodium/lithium tolerance in fission yeast. EMBO J. 11, 1631-1640. (pubmed link)
Rowley, R., J. Hudson and P. G. Young. 1992. The wee1 protein kinase is necessary for radiation induced G2 delay. Nature 356, 353-355. (pubmed link)
Hudson, J.D., H. Feilotter, C. Lingner, R. Rowley and P. G. Young. 1991. stf1: a new suppressor of the mitotic control gene, cdc25, in Schizosaccharomyces pombe. Cold Spring Harb. Symp. Quant. Biol. 56, 5999-604. (pubmed link)
Feilotter, H., P. Nurse and P. G. Young. 1991. Genetic and molecular analysis of cdr1/nim1 in Schizosaccharomyces pombe. Genetics 127, 309-318. (pubmed link)
Ducommun B, G. Draetta, P. Young,
and D. Beach. 1990. Fission yeast cdc25 is a cell-cycle regulated protein. Biochem
Biophys Res Commun 167, 301-309. (pubmed
Hudson, J., H. Feilotter and P. G. Young. 1990. Stf1: non-wee mutations epistatic to cdc25 in the fission yeast Schizosaccharomyces pombe. Genetics 126, 309-315. (pubmed link)
Molz, L., R. Booher, P. G. Young and D. Beach. 1989. cdc2 and the regulation of mitosis: six interacting mcs genes. Genetics 122, 773-782. (pubmed link)
Nasim, A., P. G. Young and B. Johnson, eds. 1989. The Molecular Biology of Fission Yeast. Academic Press, 470 pp.
Young, P. G. and P. A. Fantes. 1987. Schizosaccharomyces pombe mutants affected in their division response to starvation. J. Cell Sci. 88, 295-304. (pubmed link)
Querengesser, L. D. and Young, P. G. 1985. Changes in phosphoprotein pattern in Schizosaccharomyces pombe. Exptl. Cell Res. 159, 495-509. (pubmed link)
Young, P. G. and Fantes, P. A. 1984. Changed division response mutants function as allosuppressors in Growth, Cancer and the Cell Cycle (P. Skehan and S. Friedman, eds.), Humana Press, Clifton, New Jersey, p. 221-228.